Ag Tech and Research News

Bacterial Hijack Mechanism with Biotech Potential

Quick Share

Researchers have uncovered the intricate molecular mechanism used by parasitic phytoplasma bacteria, known for inducing ‘zombie-like’ effects in plants. This detailed revelation opens new horizons for groundbreaking applications in biotechnology and even in biomedicine.

07 December 2023, UKThe team led by Saskia Hogenhout at the John Innes Centre, in partnership with Sophien Kamoun’s group at The Sainsbury Laboratory, has employed X-ray crystallography to unveil the structure and functional mechanism of SAP05. This molecule plays a crucial role in bridging two distinct components inside plant cells.

The discovery sheds new light on a peculiar phenomenon in nature – mostly seen in “witches’ brooms” in which plant stems and leaves proliferate due to Phytoplasma bacteria.

This insect-transmitted bacteria triggers diseases like Aster Yellows, significantly diminishing yields in leaf crops including oilseed rape, lettuce, carrots, grapevines, onions, and a variety of ornamental and vegetable crops worldwide.

Phytoplasma effector SAP05 induces witches’ broom in Arabidopsis – Credit: Weijie Huang JIC

Previous research by the Hogenhout and Kamoun groups revealed how the bacterial protein SAP05 is able to manipulate plants by hijacking molecular machinery called the proteasome.

The proteasome breaks down and recycles proteins that are no longer required inside plant cells.

SAP05 hijacks this process, causing proteins which regulate growth and development to be dispensed into a molecular recycling centre known as the 26S proteasome.

This latest research focusses on how this happens at the structural level. SAP05 effectively disrupts the molecular recycling pathway, serving as a scaffold that connects its two cellular targets: a transcription factor and the proteasome.

Fascinatingly, SAP05 binds in a manner that enables it to ‘lift the dustbin lid’, selectively disposing of developmental proteins, while strategically preserving functions vital for the survival of its plant host.

Saskia explains: “We now know the structure of this complex and how the protein binds to two cellular components to create a short circuit. Whilst SAP05 allows itself to get involved in the plant, it does not disrupt other important processes. It is so amazing to see evolution crystalized in this way.”

Usually in plants, in fact across all multicellular organisms, this recycling of proteins in the proteasome is dependent on a molecule called ubiquitin.

By short-circuiting this process SAP05 provides a new way of carrying out the essential task of protein degradation which serves its own parasitic purpose and is completely independent of ubiquitin.

The discovery presents some intriguing possibilities. The researchers were struck by the sophisticated ingenuity of SAP05, a master manipulator, and its promising applications in biotechnology.

First author of the paper Dr Qun Liu said: “It was so exciting to see that this molecule SAP05 had two sides, one side binding to the transcription factor and the other binding to the 26S proteasome. It’s very smart.”

By understanding how this bacterial mechanism interacts with cells at a structural level, the researchers can now use this knowledge to engineer SAP05-like molecules which could be repurposed to remove unwanted proteins such as pathogen effectors or viruses, with an impact on therapeutics, research and in agriculture.

The work on the SAP05 effector protein continues with funding from the European Research Council, and a project led by Professor Hogenhout to investigate novel Targeted Protein Degradation (TPD) technology.

The study: Bimodular Architecture of Bacterial Effector SAP05 that Drives Ubiquitin-independent Targeted Protein Degradation, appears in PNAS.

Also Read: World Soil Day 2023: ICRISAT Leverages Innovation for Sustainable Agriculture

(For Latest Agriculture News & Updates, follow Krishak Jagat on Google News)

Quick Share